New PDF release: Adhesion of Microbial Pathogens [no cover, index]

By R. Doyle, I. Ofek

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Competition for nutrients as well as the presence of bacteriocides and predators might be important in the overall growth physiology and surface characteristics of a bacterium. Understanding the physiology of bacteria grown in vivo in various hosts has been a difficult task. By using the adhesive properties of the microorganism, it has been possible to isolate certain bacteria from their natural environment for further investigations. Pure cultures so obtained do not require growth on an artificial laboratory medium.

Labeling of the probes can be done with radioisotopes, fluorescent molecules, or biotin. Fractionation of the extracted cells into the different compartments (such as membranes, cytoplasm, periplasm) and further protein purification and characterization by conventional methods may reveal new important antigens produced in vivo only/ 1 E. A. Wadolkowski, D. C. Laux, and P. S. Cohen, Infect. Immun. 56, 1036 (1988). 2 j. p. Duguid and D. C. Old, in "Bacterial Adherence" (E. H. ), Vol. 6, p. 185. Chapman & Hall, London and New York, 1980.

Immun. 46 G E N E R A L METHODS FOR A D H E S I O N TO A N I M A L CELLS [4] Estimation of Binding of Purified Adhesins to Red Blood Cells or to Erythrocyte Membranes Binding of Red Blood Cells to Purified Adhesins (Hemadhesion). The binding capacity of RBCs to adhesins isolated from bacteria causing H A can be tested using the hemadhesion test described by Hoschtitzky et al. 14 Briefly, an RBC suspension (1% in PBS) is distributed into the wells of a microtiter plate (round bottom) in which the test adhesin has been immobilized.

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Adhesion of Microbial Pathogens [no cover, index] by R. Doyle, I. Ofek

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