Read e-book online Agrobacterium Protocols PDF

By Kevan M. A. Gartland, Michael R. Davey

Agrobacterium Protocols bargains starting and skilled researchers the main complete number of step by step protocols for the genetic manipulation of crops utilizing Agrobacterium. the themes diversity from the upkeep of bacterial tradition collections to features of the metabolism and body structure of remodeled tissues and transgenic vegetation. Drawing at the paintings of best scientists from laboratories around the globe, Agrobacterium Protocols offers a wealth of recommendations for introducing particular DNA sequences into aim plant species and discusses the environmental implications of genetically engineered crops. Its specific systems will facilitate quick move of complicated thoughts to different laboratories and their exploitation in primary and utilized plant biology.

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The vectors carrying an additional marker gene such as P-glucuronidase (GUS), chloramphenicol acetyltransferase (cat), or luciferase gene under the control of a strong plant promoter are useful for establishing a stable transformation procedure. Expression of these reporters by a strong promoter can be assayed during various stages of transformation procedure in order to optimize the transformation frequency. However, one should be careful to avoid false positive data because some plant tissues may contain endogenous enzyme activity.

Proc. Nat1 Acad. Sci. USA 87,3435-3439 17. Feldmann, K. , Marks, M. , Chnstlanson, M. , Quatrano, R. S. (1989) A dwarf mutant of Arabidopsis generated by T-DNA insertion mutagenesis. Science 243,1351-1354 18. , Van Montagu, M , and Schell, J (1983) Expresslon of chimeric genes transferred into plant cells using a Ti-plasmld-derived vector. Nature 303,209-213. 19. Ow, D. , Wood, K. , de Wet, J. , Helinski, D. , and Howell, S. H. (1986) Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants.

2,423-418 6. Palmer, A. C. V. and Shaw, C. H (1992) The role of virA and G phosphorylatlon m chemotaxts towards acetosyrmgone by Agrobacterium tumefaciens J. Gen. Microbial 138,2509-2514 7. Kerr, A. (1974) Sot1 microbiological studies on Agrobacterium radtobacter and biological control of crown gall. Soil Sci 118, 168-172. 8. , Ashby, A. M , Loake, G J , and Watson, M D (1988) One small step* the missing link m crown gall. Oxford Surv. Plant Mol. Cell Biol. 5,177-183 9. Shaw, C. H. (1991) Swlmmmg against the tide chemotaxts m Agrobactertum BioEssays 13,25-29.

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Agrobacterium Protocols by Kevan M. A. Gartland, Michael R. Davey

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