By Kevan M. A. Gartland, Michael R. Davey
Agrobacterium Protocols bargains starting and skilled researchers the main complete number of step by step protocols for the genetic manipulation of crops utilizing Agrobacterium. the themes diversity from the upkeep of bacterial tradition collections to features of the metabolism and body structure of remodeled tissues and transgenic vegetation. Drawing at the paintings of best scientists from laboratories around the globe, Agrobacterium Protocols offers a wealth of recommendations for introducing particular DNA sequences into aim plant species and discusses the environmental implications of genetically engineered crops. Its specific systems will facilitate quick move of complicated thoughts to different laboratories and their exploitation in primary and utilized plant biology.
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Extra resources for Agrobacterium Protocols
The vectors carrying an additional marker gene such as P-glucuronidase (GUS), chloramphenicol acetyltransferase (cat), or luciferase gene under the control of a strong plant promoter are useful for establishing a stable transformation procedure. Expression of these reporters by a strong promoter can be assayed during various stages of transformation procedure in order to optimize the transformation frequency. However, one should be careful to avoid false positive data because some plant tissues may contain endogenous enzyme activity.
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2,423-418 6. Palmer, A. C. V. and Shaw, C. H (1992) The role of virA and G phosphorylatlon m chemotaxts towards acetosyrmgone by Agrobacterium tumefaciens J. Gen. Microbial 138,2509-2514 7. Kerr, A. (1974) Sot1 microbiological studies on Agrobacterium radtobacter and biological control of crown gall. Soil Sci 118, 168-172. 8. , Ashby, A. M , Loake, G J , and Watson, M D (1988) One small step* the missing link m crown gall. Oxford Surv. Plant Mol. Cell Biol. 5,177-183 9. Shaw, C. H. (1991) Swlmmmg against the tide chemotaxts m Agrobactertum BioEssays 13,25-29.
Agrobacterium Protocols by Kevan M. A. Gartland, Michael R. Davey